Ląstelių ir audinių kultūrų technologija

Dalyko anotacija lietuvių kalba

Kurso metu susipažįstama su gyvūninių ląstelių ir audinių kultivavimu, darbo su gyvūninėmis ląstelėmis in vitro sąlygomis specifika ir pritaikymu biotechnologijoje. Dalyko eigoje įgyjama daug praktinių žinių, reikalingų sėkmingam ląstelių ir audinių kultūrų panaudojimui biotechnologijoje.

Dalyko anotacija užsienio kalba

The course introduces to animal cell ant tissue culture, specificity of working with animal cells under in vitro conditions and their applications in biotechnology. During this course a lot of practical knowledge, which are necessary for successful application of cell and tissue cultures in biotechnology, will be obtained.

Būtinas pasirengimas dalyko studijoms

General biology. General Genetics. Microbiology and Basics of Immunology. Instrumental Analysis. General Biotechnology.

Dalyko studijų rezultatai

Knowing and understanding the principles of cell culture techniques.
Managing to manipulate with cell cultures.
Knowing and understanding the cell culture problems and possibilities.
Knowing required conditions for cell cultures, knowing of media and the equipment required for cell culture.

Dalyko turinys

1. Introduction: The use of animal cell and tissue culture.
2. Basic equipment, facilities and chemicals required for maintaining cells in vitro. Aseptic technique
3. Conditions required for cell culture. Growth and maintenance of cells in culture.
4. Characteristics of cells in culture. Cell line and culture monitoring.
5. Contamination of cell culture.
6. Cryopreservation of animal cells. Freezing and thawing cell lines.
7. Initiation of primary cell culture.
8. Three dimensional cell culture systems.
9. Engineering of artificial tissue and organ structures for implantation and research applications.
10. Stem cells, types, properties, possibilities and perspectives.
11. Genetic engineering of animal cells in culture.
12. Hybridomas.
13. Mammalian cell products: established and potential.
14. Production from cell culture. Scaling-up of animal cell cultures
15. Ethical problems of cell culture cultivation, hazard, health care, environmental and human protection.
Practical work (contents):
Rules and specificity of working at a cell culture laboratory. The cell culture laboratory equipment, the consumables, the cell culture media and chemicals needed for cell culture. Sterile handling, maintaining sterility and aseptic technique necessary for cell culture. Subculturing of animal cells growing in a monolayer. Subculturing of animal cells growing in suspension. Initiation of primary cell culture. Growing cells in three dimensional culture. Growing the animal cells in a bioreactor.

Dalyko studijos valandomis

Lectures – 45 hours, laboratory work – 15 hours, individual work – 94 hours, examinations– 6 hours. Total 160 h.

Studijų rezultatų vertinimas

Final assessment sums the assessments of written final examination (50%), assessment of laboratory work (30%), and written mid-term examination (20%).

Literatūra

1. 2010 Freshney R. I., Culture of Animal Cells John Wiley & Sons
2. 2004 Butler M., Animal Cell Culture and Technology Garland Science
3. 2000 Freshney R. I., Culture of Animal cells Wiley-Liss
Supplementary materials
1. 2014 Pörtner R. Animal Cell Biotechnology Methods and Protocols Third Edition Edited by Humana Press (Springer Science+Business Media)
2. 1999 Doyle A., Griffiths J. B., Cell and Tissue Culture: Laboratory Procedures in Biotechnology John Wiley & Sons
3. 2013 Bettinger Ch., Borenstein J. T., Tao S. L. Microfluidic Cell Culture Systems William Andrew (imprint of Elsevier)
4. 2006 Ozturk S.S., Hu W.S., Cell Culture Technology for Pharmaceutical and Cell-Based Therapies Taylor & Francis Group
5. 2008 Castilho L.R., Moraes A.M., Augusto E.F.P., Butler M., Animal Cell Technology: From Biopharmaceuticals to Gene Therapy Taylor & Francis Group
6. 2006 Vunjak-Novakovic G. and Freshney R. I. Culture Of Cells For Tissue Engineering John Wiley & Sons