Molecular Genetics
Description
This program encompasses studies the structure and function of genes, inheritence, expression and regulation at a molecular level. Students will be introduced to useful tools and concepts that enable the study of a wide spectrum of molecular genetics; sample collection; genome scanning with subsequent linkage analysis; mutation detection methods; and the use of animal methods. All of the favorite model organisms used in modern genetic analysis are represented in this program, including phage and viruses, bacteria, yeast and fungi, nematodes, Drosophila, algae, plants, and mice. This program is rounded out by an additional strength in human molecular genetics, a research area of increasing importance..
Aim of the course
Molecular genetics is a subset of biology that examines how differences in the structure or expression of DNA molecules manifest themselves as differences in organisms. Molecular genetics often uses a "research method" to determine the structure and / or function of genes in the body's genome by genetic displays.
Prerequisites
General Biology, Biochemistry, General Genetics
Course content
1. Principles and concepts of molecular genetics
2. Gene structure and genome
3. Reverse genetics
4. Forward genetics.
5. Techniques in molecular genetics: DNA isolation, mRNA isolation, amplification, PGR, separation and detection. and expression
6. Molecular Genetics of Bacteria
7. Yeast Molecular Genetics
8. Plant Molecular Genetics
9. The Human Genome Project
10. Gene therapy
11. Cancer gGenetics
12. Epigenetic
Practical work (contents):
eucaryotic cell and tissue culture; plasmid DNA preparation; DNA amplification and analysis; genetic transofrmation; and analysis techniques.
Assesment Criteria
Be able to describe the problems of modern genetics and possible solutions; to define the role of heredity in the context of modern biology; to define the essence of molecular genetics, research methods and principles; analyze, record, compare, summarize, draw conclusions, relate subject facts to life and future profession; understand the importance of genetics in the context of the development of various sciences and society; to form an approach to the use of genetically engineered products and the significance of this use for economic progress.