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Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Navickaitė, Diana;Ruzgys, Paulius;Maciulevičius, Martynas;Chopra, Sonam;Šatkauskas, Saulius
Title: Calcium electroporation efficiency dependence on calcium concentration and administration time
Is part of: Smart Bio [elektroninis išteklius] : ICSB 3rd international conference, 02-04 May 2019, Kaunas, Lithuania : abstract book / Vytautas Magnus university. Panevėžys : UAB "Reklamos forma", 2019, Nr. 3
Extent: p. 108-108
Date: 2019
Keywords: Calcium electroporation;CHO;Cell death;Pore resealing
Abstract: Calcium ion distribution between extra and intracellular compartments plays a crucial role in cellular response to various external stress conditions. Necrotic and apoptotic processes are associated with intracellular calcium ion overload, because once calcium ions reach their threshold in the cytoplasm they cause changes in activity of enzymes, proteins and organelles ultimately leading to cell death. Intracellular calcium levels leading to cell death can be regulated by electroporation. This calcium electroporation is currently investigated as a novel anticancer treatment. In this study, we have investigated Chinese hamster ovary (CHO) cell viability dynamics after calcium electroporation. Cells were electroporated using 1 high voltage (1400 V/cm, 100 µs) electric pulse in the presence of CaCl2 at different concentrations (0; 0.25; 0.5; 1 mM) and different administration time points after cell treatment with electric pulses (0; 30; 60; 120; 240; 300; 600 s). Our results have shown that after electroporation cell viability was decreasing with increasing CaCl2 concentration and was dependent on administration time. Further evaluation of cell viability in dependence of cytotoxic agent administration time after electroporation in the presence of calcium ions shows that calcium ions can affect pore resealing dynamics
Affiliation(s): Aplinkos tyrimų centras
Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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