Universiteto mokslo publikacijos / University Research Publications

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  • conference paper ; ;
    Liaudanskas, Mindaugas
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    Žvikas, Vaidotas
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    Viškelis, Pranas
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    Viškelis, Jonas
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    Kaunas : Vytautas Magnus University, 2022
    The plants of Actinidia kolomikta are functionally dioecious so this species is characterized by both male and female flowers [1]. A. kolomikta flowers do not contain nectar and berry yields can be greatly reduced if pollinators do not visit the flowers. On the other hand, various chemical compounds in the flowers can attract pollinators. The aim of this work was to assess the diversity of phenolic compounds in male and female flowers of A. kolomikta. Separation of phenolic compounds was performed with the Acquity H-class UHPLC system (Waters, Santa Clara, CA, USA) equipped with a triple quadrupole tandem mass spectrometer (Xevo, Waters, USA) with an electrospray ionization source (ESI) to obtain MS/MS data using a previously described and validated UHPLC-ESI-MS/MS technique [2]. A total of 14 phenolic compounds were identified both in male and female flowers. Flavonols were the primary class found in flowers, other identified phenolic compounds were flavan-3-ols, phenolic acids and flavones. The major flavonols identified were kaempherol-3-O-glucoside, rutin, kaempherol, isorhamnetin, isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-glucoside, quercetin, and quercitrin. Flavon-3-ols (+)-catechin, (-)-epicatechin, and procyanidin C1 represented the second most abundant class of phenolic compounds. For flavone class, small amounts of apigenin were found. Two compounds belonging to the hydroxycinnamic acids class, i.e., chlorogenic and neochlorogenic acids were detected in both male and female flowers.
  • conference paper ;
    Duburs, Gunars
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    Vilnius : Lietuvos mikrobiologų draugija, 2022
    C. albicans yra įvardijama kaip pagrindinė padermė, sukelianti invazines kandidozes. C. glabrata sukeliamų infekcijų skaičius kasmet didėja dėl atsparumo priešgrybeliniams vaistams. Atsparumas plačiai naudojamiems azolams gali atsirasti dėl įvairių priežasčių, tarp jų ir dėl daugiavaisčio atsparumo siurblių veiklos. Naujų preparatų kūrimas, kitos paskirties medikamentų derinimas su jau žinomais priešgrybeliniais vaistais gali tapti efektyvia priemone, leidžiančia kovoti su patogeninių mielių atsparumu. Tyrimo tikslas – įvertinti naujai susintetintų stirilpiridinų klasės junginių, klinikinėje praktikoje taikomų statinų bei šių preparatų derinių su flukonazolu fungicidinį poveikį Candida mielėms bei žinduolių ląstelėms. Stirilpiridinų, atorvastatino ir flukonazolo fungicidinis poveikis C. albicans ir C. glabrata nustatytas mikroskiedimų metodu, sąveika tarp flukonazolo ir tiriamųjų junginių įvertinta nustatant frakcijos slopinančios koncentracijos indeksą (ΣFIC). Junginių poveikis CHO-K1 ląstelių gyvybingumui vertintas XTT metodu. Nustatyta, kad 0,06-8 μg/ml koncentracijų intervale stirilpiridinai efektyviai slopino C. albicans augimą, bet C. glabrata ląstelių augimo slopinimui reikėjo didesnių koncentracijų. Atorvastatino poveikis tirtoms Candida ląstelėms buvo panašus: tik 64-128 μg/ml šio junginio visiškai sustabdė augimą. Stipriausias sinerginis poveikis, efektyviai slopinantis Candida augimą, nustatytas taikant flukonazolo derinius su CSDP+ ir atorvastatinu. DEASD+ pasižymėjo didžiausiu citotoksiškumu: 2 μg/ml koncentracija visiškai nuslopino CHO-K1 ląstelių gyvybingumą. Gauti rezultatai rodo, kad stirilpiridinai ir statinai galėtų būti perspektyvūs adjuvantai, efektyviai didinantys flukonazolo aktyvumą prieš Candida mieles.
  • Canopy animals are the main carriers of mite distributed pathogens and tanks in Europe. Bison bonasus is the largest wild animal of Europe, which is recorded not only to the Lithuanian Red Book, but also to the International Red Book. For this reason, studies related to tick pathogens in the ticks gathered from animals and from spleen samples are complicated. The aim of this study is to investigate the pathogens of ticks in spleen samples and ticks collected from Bison bonasus in Lithuania. The material was collected in the territory of Lithuania in the period from 2014 year up to 2022 year from the dead (car or train accidents, dead for unknown reasons) or removed from nature by protection plan because of illness or genetic disorders. Following the analysis of the data and the analysis of the sequences found that Babesia divergens and Babesia capreoli were found in spleen samples and in the ticks collected from Bison bonasus were found Babesia divergens and Babesia divergens, Babesia mircoti and Babesia venatorum. Also, in the bison samples and the ticks were found Anaplasma phagocytophilum and Borrelia burgdorferi pathogens.
  • book-chapter ; ;
    Singapore : Springer Nature, 2022
    This chapter reviews the institutionalisation of the qualifications and training of VET teachers in the context of the key critical junctures of the skill formation which have been taking place in Lithuania since 1990. It gives an outline of the institutionalisation of the qualifications and training of VET teachers and trainers within the context of institutional change and development of skill formation in Lithuania as well as the key critical junctures, such as post-communist transformation, access to the EU and the global economic crisis of 2008–2009. This chapter provides an outline of the developments that led to the existing model of VET teachers’ qualification and training, offers an institutional model of VET teachers’ qualification and explores possible scenarios for the future. Institutionalisation of VET teachers’ qualifications in Lithuania has been strongly influenced by post-communist transformation, as well as access to the EU. Accession to the EU promoted a more holistic attitude towards VET policymakers and stakeholders regarding skill formation and qualifications. This enabled more systemic attention to the professionalisation and qualifications of VET teachers. Digitalisation of work processes, competence-based reforms of VET curricula and development of work-based learning approaches increasingly require VET teachers in Lithuania to develop their academic knowledge, professional know-how and skills as well as key skills and competencies. These factors foster dynamic institutional changes in the provision of academic and professional qualifications as well as the continuing professional development of VET teachers and trainers.
  • research article ; ;
    European studies – the review of European law, economics and politics
    The meaning of the general principles of EU law has been broadly developed by the Court of Justice of the European Union; however, for many years it had only limited competence in deciding criminal cases. The principle of direct effect is important for ensuring the efficient functioning of EU law. The aim of this research is to find out if and how this principle affects criminal justice. To reach this objective, the researchers examine how the substance and content of the principle, through the doctrine and the judgments of Court of Justice of the European Union, can influence national criminal law and criminal procedure. Afterwards, the actual impact of EU law on national criminal law is evaluated, taking Lithuania as an example. The analysis reveals that direct application of directives in material criminal law is highly unlikely, while in criminal procedural, law such a possibility is real if EU norms are clear, unconditional, and precise.