Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/56965
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dc.contributor.authorDūda, Gediminas-
dc.contributor.authorDrevinskas, Tomas-
dc.contributor.authorMaruška, Audrius Sigitas-
dc.coverage.spatialLT-
dc.date.accessioned2018-10-07T01:08:47Z-
dc.date.available2018-10-07T01:08:47Z-
dc.date.issued2017-
dc.identifier.isbn9786098104424-
dc.identifier.otherVDU02-000021567-
dc.identifier.urihttps://hdl.handle.net/20.500.12259/56965-
dc.description.abstractProtein precipitation by salts is often used method for protein purification. One of must often used salt for protein precipitation is ammonium sulfate. After protein precipitation procedure, protein solution requires method to remove salts from solution. The aim of this study was to develop on capillary electrophoresis principles based equipment to remove salts from protein solution. Cheap and easy to access parts were chosen to construct apparatus. Lysozyme solution after purification by protein precipitation procedure was used as sample to test effectiveness of apparatus. Salt removing conditions were: 0,5 M, pH=2.58 acetic acid was used as background electrolyte; capillary length – 12 centimeters; applied voltage – 2,7 kV; sample volume up to 1 ml. After salt removing procedure, sample were analyzed using capillary zone electrophoresis. Analysis conditions were; 0,5 M acetic acid was used as background electrolyte, applied voltage was 14 kV. Injection 50mbar*30s; analysis time 40 minutes. Detector type – contactless conductivity detectoren
dc.description.sponsorshipGamtos mokslų fakultetas-
dc.description.sponsorshipInstrumentinės analizės APC-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 65-65-
dc.language.isoen-
dc.relation.ispartofSmart Bio: international conference, 18-20 May 2017, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2017-
dc.subjectCapillary electrophoresisen
dc.subjectPurification by protein precipitationen
dc.subjectContactless conductivity detectoren
dc.subjectLysozymeen
dc.subject.classificationKonferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)-
dc.subject.otherBiologija / Biology (N010)-
dc.titleDevelopment of capillary electrophoresis equipment for salt removing from protein solutionsen
dc.typeconference paper-
dcterms.bibliographicCitation0-
dc.date.updated2017-11-17T14:36Z-
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local.typeT-
item.grantfulltextopen-
item.fulltextWith Fulltext-
crisitem.author.deptInstrumentinės analizės atviros prieigos centras-
crisitem.author.deptInstrumentinės analizės atviros prieigos centras-
crisitem.author.deptInstrumentinės analizės atviros prieigos centras-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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