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Effects of hyperthermia on mitochondrial respiration and NAD(P)H fluorescence
Type of publication
Tezės Web of Science duomenų bazėje / Theses in Web of Science database (T1a1)
Author(s)
LT | ||
LT | ||
LT | ||
Banienė, Rasa | Kauno medicinos universitetas | LT |
LT |
Title
Effects of hyperthermia on mitochondrial respiration and NAD(P)H fluorescence
Is part of
FEBS journal. Oxford : Blackwell Pub., Vol. 273, suppl. 1 (2006)
Date Issued
Date Issued |
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2006 |
Publisher
Oxford : Blackwell Pub
Publisher (trusted)
Is Referenced by
Extent
p. 77-77
Field of Science
Abstract
Hyperthermia has high potential as a cancer treatment modality. That implies the need to determine the kinetic response of mitochondria from healthy tissue to moderate heating as well. We have compared the effect of moderate heating on the respiration and NAD(P)H fluorescence in isolated rat heart and liver mitochondria incubated at various Ca2+ concentrations. The rise of temperature from 37 to 42 C caused substantial increase in the inner membrane permeability in both liver and heart mitochondria, but state 3 respiration in heart mitochondria increased by 30% whereas it decreased by 13–23% in liver mitochondria [NAD(P)H fluorescence did not changed in both cases]. The response of liver and heart mitochondria was very different in the range of temperature from 42 to 47 C. Complete uncoupling of oxidative phosphorylation and the inhibition of the respiration was observed at 47 C in isolated heart mitochondria. Respiration was completely ceased in liver mitochondria, indicating that their respiratory chain is more susceptible to higher temperature. Increase of temperature to 47 C was followed by NAD(P)H fluorescence decrease both in heart and liver mitochondria. Change of free Ca2+ concentration in incubation medium from granules by A. Galoyan. In the experiments 108 Wistar male rats of 160–200 g mass were used. CS was induced by a compression of femoral soft tissues using a special press. Common amount of calcium ions was defined using crezolphtalein spectrophotometer method. The results show the level of Ca2+ in blood after 2 h compression and 2, 4, 24 and 48 h decompression and under the influence of PRP. After 2 h compression the level of Ca2+ decreases by 21% , and during decompression period the concentration of Ca2+ increases in blood by 20%, 21%, 36%, 47%, accordingly after 2, 4, 24 and 48 h decompression. So, the decompression period after 2 h compression is characterized by the increasing level of Ca2+ in blood.[...].
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Jungtinė Karalystė / United Kingdom of Great Britain and Northern Ireland (GB)
Description
31st Congress of the Federation-of-European-Biochemical-Societies (FEBS) Istanbul, Turkey, Jun. 24-29, 2006 : meeting abstracts