A new approach to human serum albumin chiral stationary phase synthesis and its use in capillary liquid chromatography and capillary electrochromatography

Abstract

Continuous beds with immobilised human serum albumin as a chiral selector were synthesised in fused-silica capillaries in a modified fashion using additives during the protein allylation and polymerisation steps. Acetyl salicylic acid and L-tryptophan were used as additives to interact with the active centres that are responsible for chiral recognition, thus preserving them during the protein coupling procedure. The beds synthesised under modified conditions exhibit higher resolutions and enantioselectivities in capillary liquid chromatography, especially when L-tryptophan was used as an additive. Monolithic adsorbent synthesised with L-tryptophan possesses much higher efficiency when capillary electrochromatography was employed.