Screening of oxazepine indole enantiomers by means of high performance liquid chromatography with imprinted polymer stationary phase

Abstract

Chromatographic enantiomer separations of different oxazepine indole derivatives were performed using a molecularly imprinted polymer. A 5aR,12R,13S-trans-6,6-dimethyl-12,13-dihydro-6H-5a,13-methanoindolo[2,1-b][1,3] naphthoxazepine-12-carboxamide enantiomer derivative was used as a template and the resultant polymer has shown enantiomer recognition for series of template related compounds. The mechanistic description of the chiral discrimination process is scrutinised, comparing the discrimination between the different conformations and substituents of the oxazepine indoles.