Nowadays, as a means of supplying the market with a highly qualified workforce, vocational teachers play a significant role in Lithuania’s educational system. It is assumed that the most important factor determining the quality of education is the teacher. Undoubtedly, the vocational teacher’s personality traits and their work motivation are considered to be important components of a successful educational process. Aim: this study was carried out in order to detect the relationship between the vocational teachers’ personality traits and their work motivation. Method: The personality traits were evaluated using the Big Five Inventory (BFI; John et al., 1991, 2008) while the work motivation was assessed by employment of the Multidimensional Work Motivation Scale (MWMS; Gagne et al., 2015). 364 teachers from various Lithuania’s vocational schools participated in the research (243 of them were women, 93 men, while 10 did not specify their gender). Results: The study revealed that a greater expression of neuroticism correlated with an increase of the teachers’ motivation (p < .01), extrinsic (social and material) regulation (p < .01) and introjected regulation (p < .05), although the identified regulation (p < .05) and intrinsic motivation (p < .01) were observed to decrease. Meanwhile, with the increase of the teachers’ expression of extraversion, agreeableness and conscientiousness, intrinsic motivation (p < .01) and identified regulation (p < .01) heightened, and the extrinsic (material) motivation decreased (in case of extraversion and agreeableness – p < .01, in case of conscientiousness and openness to experience – p < .05). Moreover, the differences of work motivation noticed among various personality profiles were added to the results of the research. [...]