Continuous monitoring in football training is a very important element and it is closely connected with fitness tests. The Running-based Anaerobic Sprint Test (RAST) was developed to test an athlete’s anaerobic performance. It is similar to Wingate Anaerobic 30 cycle Test, but RAST can be used with athletes where running forms the basis of the movement. This work is aimed at defining maximal power of organisms of football players. This test was carried out in the Academy of Physical Education in Kaunas. The research has been carried out with football players of Lithuanian first division – LKKA Kaunas. After warm-up players performed six 35-meter sprint runs at maximum speed with 10 seconds allowed between each sprint for a turnaround. All of the players were monitored during the test by Sport tester Polar S610 (Heart Rate), before and after the test by dr. Lange (Lactate). Obtained results were placed in tables and subjected to statistical analysis based on calculation of arithmetic mean and standard aberration. The results of Running-based Anaerobic Sprint Test on a track provide football coaches with better information than Wingate Test, as far as running in comparison to cycling is concerned.