The article determines the level of spirituality expression of Lithuanian mid-level businesses CEO’s. The authors briefly present a scientific premise of spiritual leadership theory that was the basis for the research. Spirituality is represented in three different levels: intra-personal, supra-personal and inter-personal by identifying elements of each level that are interrelated. The article points out links with other similar scientific research, describes research methods and research instrument, provides research scope and stages, and indicates methodologically significant data collection and analysis procedures. Research findings show CEO’s spirituality expression that leads to a conclusion that spirituality among leaders of mid-level Lithuanian businesses is not expressed enough in neither of the above spirituality levels. However, the presence of high score allows us to infer that there are some instances of spiritual leadership among these executive managers.