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Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Mardosaitė-Busaitienė, Dalytė;Radzijevskaja, Jana;Voverytė, Eglė;Paulauskas, Algimantas
Title: Detection of Borrelia Species in small mammals in Lithuania
Is part of: Smart Bio: international conference, 18-20 May 2017, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2017
Extent: p. 60-60
Date: 2017
Keywords: Borrelia afzelii;Borrelia miyamotoi;Rodents;Mammals
ISBN: 9786098104424
Abstract: Borrelia is a genus of bacteria of the spirochete phylum, which causes borreliosis – a zoonotic, vector-borne disease transmitted by ticks and lice to human and animals. Small mammals are important reservoirs hosts for different Borrelia pathogens. The aim of the study was to estimate the prevalence of Borrelia spp. in different species of small mammals collected in Lithuania. A total of 241 small mammals belonged to the nine species Apodemus flavicollis (n=150), Myodes glareolus (n=34), Mus musculus (n=15), Micromys minutus (n=14), Apodemus agrarius (n=10), Microtus oeconomus (n=9), Microtus agrestis (n=4), Microtus arvalis (n=2) and Sorex araneus (n=3) were captured with live-traps in different locations of Lithuania in 2010, 2015–2016. DNR extracted from animal urine bladder was examined for the presence of Borrelia by using conventional, multiplex and nested PCR, and sequence analysis. ospA gene and 16S (rrsA) – 23S (rrlA) intergenic spacer region (ITS) were used as targets. Borrelia spp. was detected in 28 (11.7 %) of small rodents. In total, 11.7% (20/150) of Apodemus flavicollis, 5.9% (2/34) of Myodes glareolus, 2 of the 4 Microtus agrestis, one of the 2 Microtus arvalis, and 3 of the 9 Microtus oeconomus were found to be infected with Borrelia pathogens. The genotyping of ospA gene and sequence analysis of partial 16S (rrsA) – 23S (rrlA) ITS region of Borrelia indicated the presence of two Borrelia species: B. afzelii from the Borrelia burgdorferi sensu lato complex, and Borrelia miyamotoi belonging to the relapsing fever group. Phylogenetic analysis of ITS region sequences demonstrates that Borrelia isolates from small rodents are 99-100% similar to those B. afzelii and B. miyamotoi isolates derived from patients, ticks and rodents. This is the first report of B. miyamotoi found in small rodents in Lithuania
Affiliation(s): Aplinkotyros katedra
Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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