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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Chemija / Chemistry (N003)
Author(s): Maruška, Audrius;Stankevičius, Mantas;Stanius, Žydrūnas
Title: Coupling of capillary electrophoresis with reaction detection for the on-line evaluation of radical scavenging activity of analytes
Is part of: Procedia chemistry. Amsterdam : Elsevier B.V., 2010, Vol. 2, iss. 1
Extent: p. 54-58
Date: 2010
Note: 5th Symposium by Nordic Separation Science Society (NoSSS 2009); Tallinn Univ Technol, Tallinn, ESTONIA, AUG 26-29, 2009
Keywords: Antioxidant activity;DPPH;Capillary electrophoresis;On-line reaction detection
Abstract: The main task of this work was to create a rapid, simple and less material and time consuming method involving capillary electrophoretic separation in order to separate analytes and evaluate antioxidant activity within a single analytical run. Several interfaces were developed and used to couple CE to the reaction detector. The method developed enables simultaneous electrophoretic separation and evaluation of antioxidant activity of each separated compound in the mixture. The analysis was performed using DPPH (2,2-diphenyl-1-picrylhydrazyl) as synthetic radical reagent. The on-line capillary electrophoresis-reaction (antioxidant activity) detection method can be used for a rapid evaluation of individual antioxidants in complex mixtures, particularly extracts of natural products. Possibility to evaluate radical scavenging activity of extracts of natural products components is demonstrated on an example of aqueous propolis extract. Four phenolic acids where separated and their radical scavenging activity was on-line evaluated
Affiliation(s): Biochemijos katedra
Vytauto Didžiojo universitetas
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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