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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Ragauskaitė, Lauryna;Gelvonauskienė, Dalia;Haimi, Perttu;Mildažienė, Vida;Baniulis, Danas
Title: Treatment of Arabidopsis thaliana seeds with cold plasma induces changes in seedling and plant development and protein expression
Is part of: Smart Bio : ICSB 3rd international conference, 02-04 May 2019, Kaunas, Lithuania : abstract book / Vytautas Magnus university. Panevėžys : UAB "Reklamos forma", 2019, Nr. 3
Extent: p. 259-259
Date: 2019
Keywords: 2D-electrophoresis;Dielectric barrier discharge plasma;Morphology;Reactive oxygen species
Abstract: Successful seed germination and seedling establishment are critical steps in agricultural production and in the maintenance of natural ecosystems. Seed treatment cold plasma (CP), is an emerging eco-agricultural technology that has been suggested to stimulate plant growth. In addition to sterilization effect, treatment of seeds with CP has been shown to have effect on a broad spectrum of plant development and physiological processes. Although the body of information on the biological effects of the CP is growing, the complex biological mechanisms involved in the plant response remains vague. To gain an insight into the cellular processes underlying response of Arabidopsis thaliana to the CP seed treatment, seedlings and plant morphological changes, production of reactive oxygen species (ROS) and differential protein expression was assessed. The most prominent morphological effect was observed after the seed treatment for 0,5-4,5 min at 3 mm distance from the dielectric barrier discharge (DBD) plasma source at 60-80% air humidity, after the seeds were stored for 7 days at 25 oC. The treated plants had more compact rosette and longer flower stems. Assessment of ROS production in germinated seedlings using nitro blue tetrazolium and 3,3′- diaminobezidine staining revealed increase in accumulation of superoxide and H2O2. The 2Delectrophoresis analysis revealed 34 and 4 proteoforms differentially expressed after CP treatment in seedlings and leaves, respectively. Proteins involved in biological processes of response to stress and metabolism were identified
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Lietuvos agrarinių ir miškų mokslų centro Sodininkystės ir daržininkystės institutas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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