Portable Photometer for Enzymatic Measurements in situ

Abstract

Miniaturized instruments offer a possibility of using them in situ. Such approach in (bio)chemical analysis greatly reduces the time needed for an analytical task, because no sample transportation is needed resulting in faster analytical process and possibility to spoil the sample is reduced. In this work, previously developed miniaturized colorimeter has been upgraded [1]. Using the designed system, the enzymatic activity has been determined following previously published procedure [2]. The fungi were grown according to the previously optimized downscaled format [3]. In this work a design of miniaturized photometer and application determining laccase activity in malt extract fermentation medium will be discussed