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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Shakeneva, Dinara;Krokaitė, Edvina;Jocienė, Lina;Siksnian, J;Tarassovskay, N;Kupčinskienė, Eugenija
Title: Testing methods for marking loci of Lythrum salicaria for AFLP analysis
Is part of: Smart Bio [elektroninis išteklius] : ICSB 3rd international conference, 02-04 May 2019, Kaunas, Lithuania : abstract book / Vytautas Magnus university. Panevėžys : UAB "Reklamos forma", 2019, Nr. 3
Extent: p. 234-234
Date: 2019
Keywords: Lythrum salicaria;AFLP;Polymerase chain reaction;Electrophoretic detection;Genetic diversity
Abstract: The present study reports a quick, simple and inexpensive method to isolate genomic DNA suitable for AFLP analysis and other PCR-based applications. The modified CTAB protocol used in this study could be a useful protocol for extraction of high quality DNA for Lythrum salicaria. The DNA was then used for AFLP analysis. After optimization of the reaction conditions, AFLP was used to study genetic diversity among Lythrum salicaria accessions [1]. Our study was conducted within the biodiversity exploratories Kazakhstan and Lithuania. Our exploratory spans an area which study plots were established on types of to the river basin, to the river fragment state, to the land cover types of neigh bouring areas, employing 3-level hierarchy Corine classification system. The plants were examined by AFLP analysis [2]. AFLPs are dominant markers, such that an individual either has or does not have at least one allele yielding a specific amplified fragment due to modification of restriction sites or changes in neighboring selective nucleotides. The selective amplification allows one to separate the amplified DNA fragments by size. Statistical analyses of AFLP patterns were based on the ssumptions that AFLP markers are dominant markers with alleles either present or absent, and co-migrating fragments represent homologous loci [3]. Band profiles were scored on the basis of the presence or absence of strong, clear bands. Faint bands were excluded from the analysis. Total diversity was partitioned into that withinregions or populations and from this the proportion of variation that was between regions or populations was calculated
Affiliation(s): Aplinkos tyrimų centras
Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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