Effect of extract from potato tubers with coloured flesh on proliferation and viability of human gastric adenocarcinoma cells

Abstract

Introduction: Over the past decade potatoes with purple- and red-fleshed color rich in antioxidants (phenolic compounds - phenolic acids, anthocyanins etc.) have attracted the attention of consumers and scientists due to their appearance, flavour and biological properties. It is assumed that they have the potential to be an important source of biologically active compounds in the human nutrition and beneficial for human health. The aim of this study was to investigate whether potato tubers with coloured flesh might suppress human gastric adenocarcinoma cells (AGS cells) proliferation and viability. Object and methods: The field experiment was conducted in 2017 and 2018 on farm in Širvintos district (Lithuania). The study was implemented with 5 potato cultivars (Solanum tuberosum L) with different coloured flesh. In the conventional farming system the mix of universal complex fertilizers (Blaukorn Novatec N14P7K17) was used during potato crops’ vegetative period by inserting the 112 kg ha-1 nitrogen, 56 kg ha-1 phosphorus and 136 kg ha-1 potassium, as well using the pesticides according to the requirements. AGS cells were maintained in RPMI medium, supplementing 10% of fetal bovine serum and 1% penicillin / streptomycin. All cells were cultivated at 37 °C in 5% CO2 humidity. Cells were treated with extracts (0.1-50 mg dry weight/ml) for three hours, under 37 °C, 5% CO2 and humidity. After the treatment cell‘s media was changed into fresh cell media, cells viability (using MTT assay) was determined after 24 h and 72 h after the treatment. [...]