Please use this identifier to cite or link to this item:
Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Sližytė, Ramunė;Maruška, Audrius
Title: Validation procedure of 96-well plate spectrophotometric method for the quantification of flavonoids and total phenolic compounds
Is part of: Vital nature sign [electronic resource] : 13th international scientific conference, May 16-17, 2019, Kaunas, Lithuania : abstract book / editors Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus University, 2019, [no. 13]
Extent: p. 80-80
Date: 2019
Keywords: Validation;Flavonoids;Total phenolic compounds;96-well plate spectrophotometry;Automation of assay
Abstract: Validation procedure for determination of flavonoids and total phenolic compounds (TPC) was prepared for Biosan HiPo MPP-96 Microplate Photometer (Latvia). The advantage of new assay based on 96-well plate procedure is simple, fast and inexpensive measurement decreasing total assay solution volume up to 4 times in comparison with the previously optimized non-automated spectrophotometric assay using manual pipetting and spectrophotometer (Spectronic, Milton Roy, USA) [1, 2]. Calibration standards of rutin solution were prepared within the range of 0.01–1.00 mg/ml. For determination of flavonoids absorbance was measured at 407 nm (Milton Roy) and 405 nm (Biosan) and for determination of TPC was measured at 760 nm (Milton Roy) and 694 nm (Biosan). Calibration curve was built of 5 concentrations. To evaluate the linearity within described concentration range and determination coeficient R2, accuracy, precision, detection limit and quantification limit new assay was validated according to the recommendations of ICH Guideline Q2(R1) [3]. Accuracy was evaluated using known intermediate rutin solutions. The interday precision was evaluated following the same procedure for different days. The precision of the measurements was reported as the relative standard deviation (RSD%). Detection limit and quantification limit were determined based on the standard deviation of the response and on the slope of the calibration curve [3]
Affiliation(s): Gamtos mokslų fakultetas
Instrumentinės analizės atviros prieigos centras
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

Files in This Item:
marc.xml5.38 kBXMLView/Open

MARC21 XML metadata

Show full item record

Page view(s)

checked on Aug 17, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.