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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Miškotyra / Forestry (A004)
Author(s): Buchovska, Jurata;Danusevičius, Darius;Kembrytė, Rūta
Title: Investigation of the genetic origin of monument - tree The Witch Tree (Picea abies) In Rambynas Regional Park
Is part of: Smart Bio : ICSB 3rd international conference, 02-04 May 2019, Kaunas, Lithuania : abstract book / Vytautas Magnus university. Panevėžys : UAB "Reklamos forma", 2019, Nr. 3
Extent: p. 167-167
Date: 2019
Keywords: Picea abies;DNA;Microsatellites;Rambynas Regional Park
Abstract: The Witch tree consists of several different genotypes (seedlings) created by debarking them and binding together so allowing the stems to fuse the cambiums info a single stem at a later age. About the Witch tree, it is a ca. 200 years old Norway spruce tree distinguishing by the large number of mature stems (˃12); located in western Lithuania, near Šilutė in Rambynas Regional Park. We sampled wood for DNA extraction from each the 12 stems of Witch tree and used 2 trees of Picea abies from Bubliškes park as controls. The research was carried out at Vytautas Magnus university Agriculture academy laboratory of Forest genetics. 10 polymorphic microsatellite DNA loci were used to assess the genetic identity of each of the 12 stems of the Witch tree. The alleles were scored on capillary electrophoresis with ABI310. The DNA test showed that the 12 stems possess identical alleles at all 10 loci. This indicates that all 12 stems of the Witch tree belong to the same genotype, individual tree
Affiliation(s): Miškų ir ekologijos fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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