Vitaminų poveikis žieminių rapsų (Brassica napus L.) atsparumui šalčiui in vitro

Abstract

Research was carried out in the Laboratory of Agrobiotechnology of Institute of Biology and Plant Biotechnology in the Faculty of Agronomy at the Aleksandras Stulginskis university (Vytautas Magnus university Agricultural academy) in 2017 – 2018. Research in vitro was carried out with (Brassica napus L.) varieties: ‘Anderson’, ‘Kuga’, ‘Mercedes’ and ‘Visby. Isolated explants were growing on Murashige and Skoog (MS) + 30,0 g l–1 sucrose (control variant), latest transfer on MS+ 0,8 mg l–1 B1+ 1,0 mg l–1 B6 + 1,0 mg l–1 PP; MS+ 0,8 mg l–1 B1+ 1,0 mg l–1 B6 + 1,0 mg l–1 PP + 60,0 g l–1 sucrose. The result of the study shown that the frost resistance of plants in vitro depends on genotype, added vitamins and increased sucrose content in nutrient medium, wintering of variety and freezing temperatures. The result of the study confirmed that the frost resistance of plants is a genetically determined property. In plants grown at –10 °C, modified medium with vitamin supplementation and increased sucrose content, the highest (51%) survival rate was observed for the variety 'Mercedes'