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Type of publication: Straipsnis recenzuojamoje Lietuvos konferencijos medžiagoje / Article in peer-reviewed Lithuanian conference proceedings (P1f)
Field of Science: Agronomija / Agronomy (A001)
Author(s): Kisieliauskaitė, Irma
Title: Vaistinio čiobrelio netiesioginė regeneracija somatinių audinių kultūroje
Other Title: Common thyme indirect regeneration in somatic tissue culture
Is part of: Jaunasis mokslininkas 2019 [elektroninis išteklius] : studentų mokslinės konferencijos pranešimų rinkinys, 2019 m. balandžio 11 d. / Vytauto Didžiojo universitetas. Žemės ūkio akademija. Agronomijos fakultetas. Akademija, 2019
Extent: p. 87-90
Date: 2019
Abstract: The experiments were carried out at Aleksandras Stulginskis University (since 2019 at Vytautas Magnus University agricultural academy), in the Institute of Biology and Plant Biotechnology, the Laboratory of Agrobiotechnology in 2018–2019. The aim is to determine the effect of growth regulators on the dedifference of somatic tissues of thyme (Thymus vulgaris L.) in vitro. Two explant types were used: leaf and node. Explants were planted on Murashige and Skoog (MS) medium supplemented with different growth regulators concentrations and combinations: 1,0 mg 1-1 BAP, 1,0 mg l-1 BAP + 0,1 mg 1-1 IAR, 1,0 mg l-1 BAP + 0,2 mg 1-1 IAR, 1,0 mg l-1 BAP + 0,5 mg 1-1 IAR, 0,5 mg 1-1 KIN, 0,5 mg 1-1 KIN + 1,0 mg 1-1 2,4 D, 0,5 mg 1-1 KIN + 2,0 mg 1-1 2,4 D, 0,5 mg 1-1 KIN + 3,0 mg 1-1 2,4 D. The most intensive callus induction process (100%) was established in the nutrient medium supplemented with 1,0 mg l-1 BAP + 0,5 mg 1-1 IAR and 0,5 mg l-1 KIN + 3,0 mg l-1 2,4-D. Isolated node explants were more effective in formed shoots, the highest (94,86 %) the frequency was determined in the nutrient medium supplemented with 1,0 mg l-1 BAP + 0.5 mg l-1 IAR
Affiliation(s): Agronomijos fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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