Vaistinio čiobrelio netiesioginė regeneracija somatinių audinių kultūroje

Abstract

The experiments were carried out at Aleksandras Stulginskis University (since 2019 at Vytautas Magnus University agricultural academy), in the Institute of Biology and Plant Biotechnology, the Laboratory of Agrobiotechnology in 2018–2019. The aim is to determine the effect of growth regulators on the dedifference of somatic tissues of thyme (Thymus vulgaris L.) in vitro. Two explant types were used: leaf and node. Explants were planted on Murashige and Skoog (MS) medium supplemented with different growth regulators concentrations and combinations: 1,0 mg 1-1 BAP, 1,0 mg l-1 BAP + 0,1 mg 1-1 IAR, 1,0 mg l-1 BAP + 0,2 mg 1-1 IAR, 1,0 mg l-1 BAP + 0,5 mg 1-1 IAR, 0,5 mg 1-1 KIN, 0,5 mg 1-1 KIN + 1,0 mg 1-1 2,4 D, 0,5 mg 1-1 KIN + 2,0 mg 1-1 2,4 D, 0,5 mg 1-1 KIN + 3,0 mg 1-1 2,4 D. The most intensive callus induction process (100%) was established in the nutrient medium supplemented with 1,0 mg l-1 BAP + 0,5 mg 1-1 IAR and 0,5 mg l-1 KIN + 3,0 mg l-1 2,4-D. Isolated node explants were more effective in formed shoots, the highest (94,86 %) the frequency was determined in the nutrient medium supplemented with 1,0 mg l-1 BAP + 0.5 mg l-1 IAR.