Kvapiosios pasifloros regeneracija in vitro

Abstract

Investigation was caried out at the Aleksandras Stulginskis university, Biology and plant biotechnology institute and laboratory of Agrobiotechnology in 2014–2015. The aim of investigation was to estimate growth regulators effect on purple passionfruit shoot formation. For shoot induction were used explants of purple passionfruit stems. In the experiment were analysed effect of different cytokinins and auxins concentrations – 0,5 mg l-1 BAP; 1,0 mg l-1 BAP; 2,0 mg l-1 BAP; 0,5 mg l-1 BAP+0,1 mg l-NAA; 0,5 mg l-1 BAP+0,2 mg l-1 NAA; 0,5 mg l-1 BAP+0,5 mg l-1 NAA; 1,0 mg l-1 BAP+0,1 mg l-1 NAA; 1,0 mg l-1 BAP+0,2 mg l-1 NAA; 1,0 mg l-1 BAP+0,5 mg l-1 NAA; 2,0 mg l-1 BAP+ 0,1 mg l-1 NAA; 2,0 mg l-1 BAP+0,2 mg l-1 NAA; 2,0 mg l-1 BAP+0,5 mg l-1 NAA. The results of this study shows that the concentration of cytokinins and auxins in medium is very important for shoot regeneration. The medium supplement by 2,0 mg l-1 BAP+0,5 mg l-1 NAA is the most suitable for purple passionfruit. In this media was highest shoot formation frequency (100 %) and the highest number of shoots per explant (8,7 unit).