Kvapiojo baziliko ląstelių dediferenciacija in vitro

Abstract

The objective of this study was to investigate the effect of cytokinins concentration on callus induction in sweet basil leaves culture. The experiments were carried out in the Institute of Biology and Plant Biotechnology and JRC Laboratory of Agrobiotechnology of Aleksandras Stulginskis University in 2015–2016. Sweet basil cultivars ‘Thai’ and ‘Lemon’ have been investigated. MS medium were supplemented with different concentration of BAP and KIN, 6 % sucrose and 0.6 % agar. Under influence of BAP and KIN explants of cultivar ‘Thai’ formed callus with higher frequency in comparison to cultivar ‘Lemon’. For dedifferentiation induction from leaves segments of cultivar ‘Thai’ MS medium must be supplemented by 0.5–2.0 mg 1-1 BAP and 1.5–2.0 mg l-1 KIN. For callus induction from somatic explants of cultivar ‘Lemon’ more suitable are MS media supplemented by 2.0–2.5 mg 1-1 BAP