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Type of publication: Straipsnis nerecenzuojamoje Lietuvos konferencijos medžiagoje / Article in non-peer-reviewed Lithuanian conference proceedings (P2c)
Field of Science: Agronomija / Agronomy (A001)
Author(s): Donec, Mark
Contributor(s): Blinstrubienė, Aušra
Title: Augimo reguliatorių poveikis Fragaria L. kaliaus genezei lapkočių kultūroje
Other Title: Effect of growth regulators on Fragaria L. callus genesis in petiole culture
Is part of: Jaunasis mokslininkas 2016 [elektroninis išteklius] : studentų mokslinės konferencijos pranešimų rinkinys, 2016 m. balandžio 14 d. / Aleksandro Stulginskio universitetas. Agronomijos fakultetas. Akademija, 2016
Extent: p. 121-123
Date: 2016
Abstract: The paper presents results of the study that was carried out at Aleksandras Stulginskis University, Biology and Plant Biotechnology Institute and Agrobiotechnology laboratory in 2015–2016. The aim of this study was to estimate the effects of cytokinins on callus induction in woodland strawberry (Fragaria vesca L.) 'Regina' and 'Rugia' cultivars. Petioles of Fragaria vesca L. cultivars 'Regina' and 'Rugia' were used as explants. The sterilization was carried out by using distilled water, 10% (v/v) sodium hypochlorite (NaOCl) and 70% (v/v) ethanol aqueous solutions. Sterile explants were cultured on Murashige and Skoog's (MS) medium supplemented with cytokinin 6-benzyladenine (BAP) (0,5– 4,0 mg·l-1) and thidiazuron (TDZ) (0,5–4,0 mg·l-1). 60 explants of petioles were cultivated in each treatment. The experiment was independently replicated three times. It was found that the frequency of callus formation was higher when TDZ was applied rather than the same concentration of cytokinin BAP. Established that 'Rugia' was more effective on callus formation than 'Regina' cultivar
Affiliation(s): Vytauto Didžiojo universitetas
Žemės ūkio akademija
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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