Augimo reguliatorių poveikis Fragaria L. kaliaus genezei lapkočių kultūroje

Abstract

The paper presents results of the study that was carried out at Aleksandras Stulginskis University, Biology and Plant Biotechnology Institute and Agrobiotechnology laboratory in 2015–2016. The aim of this study was to estimate the effects of cytokinins on callus induction in woodland strawberry (Fragaria vesca L.) 'Regina' and 'Rugia' cultivars. Petioles of Fragaria vesca L. cultivars 'Regina' and 'Rugia' were used as explants. The sterilization was carried out by using distilled water, 10% (v/v) sodium hypochlorite (NaOCl) and 70% (v/v) ethanol aqueous solutions. Sterile explants were cultured on Murashige and Skoog's (MS) medium supplemented with cytokinin 6-benzyladenine (BAP) (0,5– 4,0 mg·l-1) and thidiazuron (TDZ) (0,5–4,0 mg·l-1). 60 explants of petioles were cultivated in each treatment. The experiment was independently replicated three times. It was found that the frequency of callus formation was higher when TDZ was applied rather than the same concentration of cytokinin BAP. Established that 'Rugia' was more effective on callus formation than 'Regina' cultivar