Citokinino BAP poveikis anyžinio lofanto kaliaus indukcijai in vitro

Abstract

The investigation were caried out in the laboratory of Agrobiotehnology of Aleksandras Stulginskis university. For the studies, were selected Agastache foeniculum plants. The effects of cytokinin 6-benzylaminopurine (BAP) Agastache foeniculum callus induction were investigated, of two explants: leaf and cotyledons. The segments do not formed callus in medium without growth regulators. The most intensive induction of callus by cotyledons in medium with 0.5 mg l-1 BAP, 1.0 mg l -1BAP, 1.5 mg 1-1BAP and 2.0 mg 1 -1BAP, here callus formation was from 80 till 95 %. The most intensive callus genesis was observed on leaf segments in medium supplemented with 2.5 mg 1-1 BAP. The leaf explants formed callus on average 31 % rate. The best callus induction determinated from Agastache foeniculum cotyledons explants, which responds effectively