Vaistinio šalavijo kaliaus indukcija lapų kultūroje

Abstract

The objective of this study was to evaluate influence of growth regulators combination in nutrient medium on sage (Salvia officinalis L.) callus induction in somatic tissue culture. The experiments were carried out in the laboratory of Agrobiotechnology at the institute of Biology and Plant Biotechnology in the faculty of Agronomy at the Aleksandras Stulginskis university in 2017–2018. The leaf explants were planted on Murashige and Skoog (MS) nutrient medium supplemented with different plant growth regulators. Frequency of callus formation (%) was evaluated. The study showed that using kinetin in the nutritional medium stimulated genesis of callus, but the rate of it was not significantly different. It was determinated that increasing combination of cytokinin kinetin and auxins naphthylacetic acid, increased the rate of callus formation. Tests with TDZ + NAA indicated that the most efficient use of additive substances was when the nutrient medium added with 0.3 mg l-1 TDZ + 0.1 mg l-1 NAA. The most intensive callus induction process in the salvia officinalis leaf culture was carried out in a nutrition medium supplemented with 0.25 mg l-1 KIN + 0.15 mg l-1 NAA.