Endogeninių bei egzogeninių veiksnių įtaka kvapiojo baziliko kaliaus indukcijai in vitro

Abstract

The purpose of this study was to investigate the effect of growth regulators and components of nutrition medium on callus induction of sweet basil in the culture of cotyledon. The experiments were carried out in the Institute of Biology and Plant Biotechnology and the JRC Laboratory of Agrobiotechnology of Alexandras Stulginskis University in 2017–2018. Sweet basil cultivars ‘Thai’, ‘Lemon’ and ‘Italiano Classico’ have been investigated. B5 medium was supplemented with different concentrations of 6-benzylaminopurine (BAP) and/or α-naphthylacetic acid NAA, 20 – 30 g l-1 sucrose. Among tested genotypes isolated explants of genotype ‘Thai’ formed callus in the highest frequency. Callus formation frequency varied from 9.1 till 100 % depending on genotype and components of nutrition medium interaction. For induction of somatic cells dedifferentiation of sweet basil in the culture of cotyledon B5 nutrition medium must be supplemented with 30 g l-1 sucrose and 0,5 mg l-1 BAP + 0,5 mg l-1 NAA