Eksplanto tipo įtaka paprastosios portulakos (Portulaca oleracea L.) netiesioginei organogenezei

Abstract

Investigations were carried out during 2017–2018 in the JRC laboratory of Agrobiotechnology and institute of Biology and plant biotechnology in the faculty of Agronomy at the Aleksandras Stulginskis University. The aim of the research was to evaluate the hormone influence of different explant types of Portulaca oleracea L., using different combinations and concentrations of growth regulators. Two explant types were used: cotyledon and hypocotyl. Explants were planted on Murashige and Skoog (MS) medium supplemented with different growth regulators concentrations and combinations: 1,0 mg l-1 BAP + 1,0 mg l-1 IAA; 1,5 mg l-1 BAP + 1,0 mg l-1 IAA; 2,0 mg l-1 BAP + 1,0 mg l-1 IAA; 1,0 mg l-1 BAP; 2,0 mg l-1 BAP; 1,0 mg l-1 KIN and 2,0 mg l-1 KIN. It was found that the more intense indirect organogenesis was on cotyledon explant culture.