Abiotinio streso įtaka maistui daiginamų lęšių cheminei sudėčiai

Abstract

Seed germination is one of the most effective processes for improving the quality of beans. The quality and amount of the seeds during germination changes. The most chemical content components of germinated seeds become more easily absorbed by human body. Moreover vitamins and some other compounds that can be considered useful as antioxidants are often also affected by germination. The object of the experiment is germinated edible lentils seeds (Lens culinaris L.). Amount of dry matter, vitamin C, crude proteins and flavonoids in the seeds were determined during the investigation. Seeds were germinated for 5 days (120 hours), periodically (every 12 hours) were watered with drinking water. After two days of germination, were made such conditions of abiotic stress: temperature stress - the seeds were kept at a temperature of 35°C, 40°C, 45°C for two hours; osmotic stress - the seeds were watered with 50 mM, 100 mM, 150 mM NaCl solution for 24 hours; oxidative stress - the seeds were watered with a solution of 100 mM, 150 mM, 200 mM H2O2 for 24 hours. Thus, the highest amount of dry matter was determined in the seeds germinated without stress conditions and germinated at 40 ° C for 2 hours, 24.4 and 24.7 %, respectively. Only osmotic stress (100 mM NaCl solution) substantially affected the amount of crude proteins in germinated lentils. The difference of amount of crude proteins treated with 100 mM NaCl and in stress-free lentils was 2.4 %. Other abiotic stressors did not have a significant effect on the amount of crude proteins in germinated lentils. The highest vitamin C content was in germinated lentils exposed to stress under watering with 150 mM concentration H2O2 solution. The highest amount of flavonoids was determined in germinated lentils watered with NaCl solution of 150 mM concentration (3.4 times higher than the untreated control lentils)