Factors Affecting Zantedeschia Spreng. Dedifferentiation In Vitro

Abstract

Zantedeschia Spreng. is an economically important ornamental plant species. Improvement of the plant’s aesthetic parameters and creation of novel variation of decorative plants are important economic goals for the commercial ornamental industry. The effect of growth regulators combination and explant type on dediferentation induction from Zantedeschia aethiopica and Zantedeschia elliottianna was investigated. Research was carried out in Institute of Biology and Plant Biotechnology of Aleksandras Stulginskis University and Laboratory of Agrobiotechnology of Join Research Center in 2016–2017. Explants (leaf discs, spathe discs and petiole segments) of were cultured on MS medium supplemented with different concentrations of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 6-benzylamino purine (BAP), 30.0 g l-1 sucrose and 8.0 g l-1 agar. In the medium without growth regulators leaf disc and spathe discs did not show any response, while 16.3 % (Zantedeschia aethiopica) and 24.3 % (Zantedeschia elliottianna) of isolated petiole segments formed callus. Our results showed that appropriate growth regulators combination for callus induction varied dependent on genetic background and explant type. It was documented that isolated petiole segments of the arum lily and calla lily induced statistically reliable more callus in a medium supplemented with combination BAP + IAA, while combination BAP + IBA promoted callus formation from spathe discs tissues. Petiole segments manifested the highest dedifferentiation capability among the tested explant types. The results of the study showed that somatic tissues of arum lily and calla lily ability to induce dedifferentiation seems to be a valuable material for improvement of ornamental values in this plants.