Green algae Ankistrodesmus fusiformis cell disruption using different modes

Abstract

Two different microalgae cell disruption modes, ultrasonication (VCX 130 ultrasonic processor and enclosed system) and ultrahomogenization, have been used in this study. The objective of this study was to estimate the efficiency of algae cell disruption by applying various techniques for disrupting green algae Ankistrodesmus fusiformis and using non-treated and frost-treated algae suspensions. The experiments were carried out using working regimes from 1 to 70 min. The highest efficiency (100%) of cell disruption was achieved with microalgae Ankistrodesmus fusiformis using an ultrasonic processor at the following conditions: frozen and defrosted algae suspension; fixed temperature of suspension; processing time – 60 min. Pre-treatment of microalgae suspension using frost improves disruption effectiveness. Pre-treatment of suspension using frost before mechanical cell disruption improves efficiency app. from 1 to 12%.