Stevijos kaliaus genezė lapų kultūroje

Abstract

The main aim of the research was to evaluate the influence of various growth regulators on callus formation from stevia leaf explants. Investigations were carried out in 2015–2016 at Aleksandras Stulginskis University, Faculty of Agronomy, Institute of Biology and Plant Biotechnology and JRC Laboratory of Agrobiotechnology. Leaf explants were planted on Murashige and Skoog (MS) medium supplemented with different growth regulators concentrations and combinations: BAP – 0.5 mg l-1, 1.0 mg l-1, 1.5 mg l-1, 2.0 mg l-1, 2.5 mg l-1; also KIN – 0.5 mg l-1, 1.0 mg l-1, 1.5 mg l-1, 2.0 mg l-1, 2.5 mg l-1 and different auxins IAA: 0.5 mg l-1, 1.0 mg l-1, 2.0 mg l-1 and NAA: 0.5 mg l-1, 1.0 mg l-1, 2.0 mg l-1. It was found that the most intensive callus induction were in a nutrient medium supplemented with 2.0 mg l-1 BAP, callus formation frequency – 87.3 %. In order to obtain the best callus formation in stevia leaf explants is appropriate to use the media, supplemented with 1.0 mg l-1 NAA + 2.0 mg l-1 BAP and 2.0 mg l-1 NAA + 2.0 mg l-1 BAP combinations (100 %). Nutrient medium with cytokinin 2.0 mg l-1 BAP supplement stevia leaf explants vigorously promote callus induction than the addition of 1.0 mg l-1 KIN.