Margalapės aktinidijos (Actinidia kolomikta Maxim.) organogenezės indukcija in vitro

Abstract

Research was investigated at the Laboratory of Agrobiotecnology and Institute of Biology and Plant Biotecnology at the Faculty of Agronomy in Aleksandras Stulginskis University in 2015–2016. Research in vitro was carried out with apical meristem shoots of Actinidia kolomikta Maxim. ‘Paukštės Šakarva᾽ and ‘Landė᾽. Isolated explants ware grown on (MS) Murashige and Skoog nutrition medium without growth regulators and supplemented with different content of growth regulators: BAP 1.0–5.0 mg l-1 and NAA 0.1 mg l-1 concentrations. Formation frequency (%) of roots and additional buds and additional buds number per explant ware estimated after four weeks of growing. In purpose of induction of Actinidia kolomikta ‘Paukštės Šakarva᾽ and ‘Landė᾽ rhizogenesis it is best to supplement nutrient medium with 5.0 mg l-1 BAP + 0.1 mg l-1 NAA. Formation of additional buds of apical shoots culture can be initiated by supplementing medium with 3.0 mg l-1 BAP + 0.1 mg l-1 NAA for ‘Paukštės Šakarva᾽ and with 4.0 mg l-1 BAP + 0.1 mg l-1 NAA for ‘Landė᾽