Levandos somatinių audinių dediferenciacija in vitro

Abstract

Research was investigated at the Laboratory of Agrobiotecnology and Institute of Biology and Plant Biotecnology at the Faculty of Agronomy in Aleksandras Stulginskis University in 2015–2017. Research in vitro was carried with two of Lavandula species: Lavandula angustifolia Mill. and Lavandula stoechas. The aim of the study was to evaluate the influence of growth regulators, lavender dedifferentiation somatic tissues in vitro. During the study were used shoots, stems and leaf explants. Isolated explants were growing on MS medium and were supplemented with different cytokinin concentration of BAP 0.5–5.0 mg l-1. L. angustifolia Mill. and L. stoechas lavender species dediferenciation of somatic tissue explant depended on the type and concentration of cytokinin BAP in nutrient medium. Isolated lavender shoot explants callus formation intensively 92.33 to 100 % frequency. The callus induction process in the stem segments culture was the best in medium supplemented with 3.0 to 4.0 mg l-1. BAP and L. stoechas BAP 4.0–5.0 mg l-1. Lavender leaf callus induction in tissue culture, most took place in the medium supplemented with BAP 2.0–3.0 mg l-1