Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/90912
Type of publication: Straipsnis nerecenzuojamoje Lietuvos konferencijos medžiagoje / Article in non-peer-reviewed Lithuanian conference proceedings (P2c)
Field of Science: Agronomija / Agronomy (A001)
Author(s): Stanevičiūtė, Giedrė
Contributor(s): Masienė, Ramunė
Title: Kvapiojo rozmarino ir paprastojo raudonėlio kaliaus indukcija in vitro
Other Title: Callus induction of rosemary and oregano in vitro
Is part of: Jaunasis mokslininkas 2017: studentų mokslinės konferencijos pranešimų rinkinys / Aleksandro Stulginskio universitetas. Agronomijos fakultetas. Akademija,, 2017
Extent: p. 140-143
Date: 2017
Abstract: The objective of this study was to evaluate influence of growth regulators combination and sucrose concentration in nutrient medium on rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgaris L.) callus induction in somatic tissue culture. The experiments were carried out in the laboratory of Agrobiotechnology and institute of Biology and Plant Biotechnology in the faculty of Agronomy at the Aleksandras Stulginskis university in 2016–2017. Leaf and stem explants were planted on Murashige and Skoog (MS) nutrient medium supplemented with different plant growth regulators and sucrose concentrations. Callus formation frequency (%) was evaluated. The results of this research indicates that combination of cytokinins and auxins in nutrient medium is very important for callus induction. The most intensive rosemary and oregano callus formation frequency was determined in medium supplemented with 0.5 mg l-1 BAP+1.0 mg l-1 NAR. It was found that stem segments were better explants for callus induction from both plants. Also, it‘s important to note, that higher (5 %) sucrose concentration in medium stimulated Rosmarinus officinalis L. callogenesis, but for Origanum vulgare L. tissue dedifferentiation it wasn’t a relevant factor
Internet: https://hdl.handle.net/20.500.12259/90912
Affiliation(s): Vytauto Didžiojo universitetas
Žemės ūkio akademija
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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