Kvapiojo rozmarino ir paprastojo raudonėlio kaliaus indukcija in vitro

Abstract

The objective of this study was to evaluate influence of growth regulators combination and sucrose concentration in nutrient medium on rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgaris L.) callus induction in somatic tissue culture. The experiments were carried out in the laboratory of Agrobiotechnology and institute of Biology and Plant Biotechnology in the faculty of Agronomy at the Aleksandras Stulginskis university in 2016–2017. Leaf and stem explants were planted on Murashige and Skoog (MS) nutrient medium supplemented with different plant growth regulators and sucrose concentrations. Callus formation frequency (%) was evaluated. The results of this research indicates that combination of cytokinins and auxins in nutrient medium is very important for callus induction. The most intensive rosemary and oregano callus formation frequency was determined in medium supplemented with 0.5 mg l-1 BAP+1.0 mg l-1 NAR. It was found that stem segments were better explants for callus induction from both plants. Also, it‘s important to note, that higher (5 %) sucrose concentration in medium stimulated Rosmarinus officinalis L. callogenesis, but for Origanum vulgare L. tissue dedifferentiation it wasn’t a relevant factor