Genotipo ir augimo reguliatorių įtaka pluoštinių linų organogenezei

Abstract

Research was investigated at the JRC of Laboratory of Agrobiotechnology, Institute of Biology and Plant Biotechnology, Faculty of Agronomy at the Aleksandras Stulginskis University during 2015–2016. The objective of this study was to investigate fiber flax (Linum usitatissimum L.) morphogenesis by using hypocotyls and peeled hypocotyls explants. Explants were planted on Murashige and Skoog (MS) nutrient medium supplemented with different plant growth regulators concentrations. The results of this research indicates, that growth regulators combinations stimulated shoots formation from flax explants, but the main factors influencing morphogenesis were genotype and explant type. Among investigated genotypes, somatic tissues of cultivars ‘Snaigiai’ and ‘Audriai’ manifested the best morphogenic capability. It was found, that peeled hypocotyl tissues were better explants for shoot formation. The growth regulators combination 1.5 mg l-1 BAP + 0.5 mg l-1 NAA was the most suitable for Linum usitatissimum L. cultivars