Eksplanto tipo įtaka pelargonijos kaliaus formavimuisi in vitro

Abstract

The paper presents the results of the research on the influence of explant type on geranium callus formation in vitro. Investigations were carried out at the JRC laboratory of Agrobiotechnology at the Faculty of Agronomy, Institute of Biology and Plant Biotechnology of Aleksandras Stulginskis University in the period of 2016–2017. The aim of the research was to identify the influence of different species of geranium explant types on the rate of callus formation in different combinations and concentrations of growth regulators. Two geranium cultivars were used: peltatum ‘F2 Mix’ and zonale ‘Night Violet’. For investigation of induction of callus were used explants of leaf, stem segments and petiole. Explants were planted in the following media: MS (–) and MS, by adding 1.0 mg l-1 KIN + 0.5 mg l-1 NAA; 2.0 mg l-1 KIN + 0.5 mg l-1 NAA; 1.0 mg l-1 BAP + 0.5 mg l-1 NAA and 2.0 mg l-1 BAP + 0.5 mg l-1 NAA. Callus formation rate (percent) was evaluated. The evaluation of the results showed that leaf and stem segment explants are more suitable for both cultivars of geranium callus formation in vitro than petioles. It is advisable to use stem segment explants for the two cultivars of callus formation when using nutrient medium without growth regulators. Isolated leaf explants of ‘Night Violet’ cultivar formed callus in 100 percent frequency in media with 2.0 mg l-1 + KIN 0.5 mg l-1 NAA, and the stem segments of cultivar ‘F2 Mix’ grown on nutrient medium supplemented with 2.0 mg l-1 BAP + 0.5 mg l-1 NAA.