Sedum L. regeneracija in vitro

Abstract

Investigation was carried out at the Aleksandras Stulginskis university, Biology and plant biotechnology institute and laboratory of Agrobiotechnology in 2012–2013. The experiments were carried out with four Sedum cultivars: ‘Angeline’, ‘Autum joy’, ‘Cristatum’ and ‘Elegans’. For investigation of induction on shoots were used explants of steam segments. In the experiment were analysed effect of different cytokinins and auxins concentrations (0,75 mg 1-1 2iP + 0,5 mg 1-1 NAA; 1,5 mg 1-1 2iP + 0,5 mg 1-1 NAA; 3,0 mg 1-1 2iP + 0,5 mg 1-1 NAA; 4,0 mg 1-1 2iP + 0,5 mg 1-1 NAA) on shoots formation in vitro. These studies established that, depending on a cultivar in the medium without growth regulators isolated Sedum stem segments formed shoots from 7.1 to 18.8 % frequency. Growth regulators combination 4.0 mg 1-1 2iP + NAA 0.5 mg 1-1 influenced the greatest number of shoot formation isolated explants of ‘Autum Joy’ and ‘Elegant’ cultivars. Investigated cultivar ‘Angeline’ shoot formation frequency mostly stimulated of 3.0 mg 1-1 2iP + NAA 0.5 mg 1-1 growth regulator combinations, and a cultivar of ‘Cristatum’ – combinations of 3.0 mg 1-1 2iP + 0.5 mg 1-1 NAA and 4.0 mg 1-1 2iP + NAA 0.5 mg 1-1.