Veiksniai, lemiantys Chrysanthemum L. pridėtinių pumpurų formavimąsi in vitro

Abstract

Investigations were carried out during 2012–2013 in the laboratory of Agrobiotechnology and Biology and Plant Biotechnology Institute of Aleksandras Stulginskis University. The effect of culture media and genotypes on adventitious buds regeneration in four chrysanthemum cultivars was investigated. The current study indicates that there is a strong genotype effect on adventitious buds regeneration from chrysanthemum stem segments. Among investigated genotypes, the somatic tissue of cultivar ‘Capitola’ manifested the best morphogenic capability. The growth regulators combination 1.0 mg l-1 KIN + 0.1 mg l-1 NAA was the most suitable for cultivars ‘Amiko Yellow’, ‘Garnet King’ and ‘Baltica’ buds induction, while for cultivar ‘Capitola’ 1.0 mg l-1 KIN + 0.1 mg l-1 NAA can be successfully replaced by 1.0 mg l-1 KIN + 0.1 mg l-1 IAA