Augimo reguliatorių poveikis lelijų mikrosvogūnėlių indukcijai in vitro

Abstract

Investigation was carried out at the Aleksandras Stulginskis University, Biology and plant biotechnology institute and laboratory of Agrobiotechnology in 2012–2013. The experiments were carried out with four lilies cultivars ‘Red Beauty’, ‘Wiener Blut’, ‘No Limit’ and ‘Sėkmė’. For investigation of induction on microbulbs were used explants of bulbs scales. In the experiment were analysed effect of different cytokinins and auxins concentrations (1.0 mg l-1 BAP + 0.3 mg l-1 NAA; 2.0 mg l-1 BAP + 0.3 mg l-1 NAA; 3.0 mg l-1 BAP + 0.3 mg l-1 NAA) on microbulb formation in vitro. The current study indicates that the lilies microbulbs formation rate in vitro determined by growth regulators and genotype interaction. The same cultivars of lilies are characterized by different regenerative capacity, depending on the nutrient medium composition. Among investigated cultivars the highest morphogenic potention distinguished isolated explants of ‘Red Beauty’. For micropropagation of cultivar ‘No Limit’ most suitable is medium supplement by 1.0 mg l- 1 BAP + 0.3 mg l-1 NAA combination. All isolated explants of cultivar ‘Sėkmė’ formed microbulbs on medium with 2.0 mg l-1 BAP + 0.3 mg l-1 NAA and 3.0 mg l-1 BAP + 0.3 mg l-1 NAA. Growth regulators combination 3.0 mg l-1 BAP + 0.3 mg l-1 NAA influenced cultivars ‘Red Beauty’ and ‘Wiener Blut’ microbulb formation in highest frequency.