Augimo reguliatorių poveikis žaliosios šerytės kaliaus indukcijai in vitro

Abstract

Investigations were carried out during 2011-2012 in the Laboratory of Agrobiotechnology of Aleksandras Stulginskis University. Experiments were grown at 60 explants of each option; the study was performed in three replications. Every four weeks, explants were transferred to fresh the same composition nutrient medium. The formation rate for callus was evaluated (%). Dedifferentiation process of isolated green millet tissue began 7-10 days after isolation of leaf segments. The least frequency (4.44 percent) of callus were formed in medium with 2 mg l -1 2,4 D + 0.2 mg l-1 BAP. In medium, which was dominated by cytokinins callus formation frequency was very low. The highest frequency of callus formation (55 percent) of green millet leaf segment cultures were when explants was cultivated on medium supplemented by 2 mg l-1 2,4 D + 0.4 mgl-1 KIN.