Genotipo įtaka linų morfogenezės procesui izoliuotų dulkinių kultūroje
Date |
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2012 |
Research was carried out at the Laboratory of Agrobiotechnology of Lithuanian University of Agriculture in 2010- IIll. Genotype, which influence morphogenesis in linseed flax cultivars 'Lirina', 'Barbara', 'Mikaei', 'Atalante', Norman', 'Dnepr-2' in anthers culture have been investigated. There were analyzed different genotypes anthers, which where grown in media differing in their levels of growth regulators and sugars concentration. Results show that, linseed flax lIus organogenesis intensity depends not just from medium, but also from genotype. Genotypes 'Mikaei' and 'Dnepr-2' ndiers formed callus statistically reliable intensive formed shoots in medium with 2,0 mg l"1 BAP + 1,0 mg 1"! NAA. The maximum number of shoots per explant tissue was obtained in the second subculture.