Egzogeninio prolino poveikis žieminio rapso ūglių atsparumo šalčiui in vitro

Abstract

The objective of this study was to investigate the effect of exogenous proline added to medium on rapeseed shoots cold tolerance in vitro. The experiments carried out in the laboratory of Agrobioteclmology of Aleksandras Stulginskis University in 2010-2011. Increasing the concentration of exogenous proline in a nutrient medium resulted in increases of endogenous proline content in winter oilseed rape 'Sunday' shoots. The biggest soluble carbohydrate content in winter rapeseed shoots as determined by the cultivation of nutrient medium containing 10 M proline. Prolonged de- acclimation duration increased electrolyte conductivity of winter rape shoots. Increasing of proline concentration in nutrient medium resulted in decreases of electrolyte leakage