Genotypic and growth regulator effects on organogenesis from hypocotyl explants of fiber flax (Linum usitatissimum L.)

Abstract

The species Linum usitatissimum L. has been the focus of a great deal of both basic and applied research effort in plant cell and biotechnology studies in recent years. The effect of culture media and genotypes on adventitious buds regeneration in five fiber flax cultivars was investigated. Hypocotyls of the tested genotypes were induced to form callus by culturing on the Murashige-Skoog medium supplemented with different concentrations of 6- benzylaminopurine (BAP), 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ), N6-(2-isopentyl) adenine (2iP) and 1-naphthylacetic acid (NAA). Adventitious buds were regenerated from the organogenic callus on the same medium in all types of tested media. The current study indicates that there is a strong genotype effect on adventitious buds regeneration from fibre flax hypocotyls, and therefore specific combination of growth regulators must be designed for each genotype. Among investigated genotypes, the hypocotyls of cultivar ‘Vaižgantas’ manifested the best morphogenic capability.