The paper describes results of investigations of biodiesel fuel production process using bioca-talysts. Effectiveness of industrial lipases in the process of esterification was investigated and the most effective biocatalysts - Novozym 435 and Lipozyme RM IM were selected. Optimal conditions of esterification are presented. Possibilities to apply multi-stage esterification process by using methanol sensitive ferment preparation Lipozyme RM IM were evaluated and data about effectiveness of bioca-talyst depending on process conditions (temperature, quantity of catalyst, molar ratio of methanol to vegetable oil) is presented. Quality analysis of product obtained applying multi-stage transesterification process showed that transesterification process have to be performed applying continues flow process. Results of studies of effectiveness of various industrial adsorbents for glycerol elimination from reaction medium are presented. Technological scheme of continuous biodiesel fuel production from raw materials containing high amount of free fatty acids by using biocatalysts lipases and performing esterification and transesterification processes simultaneously is presented.
