Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/60812
Type of publication: conference paper
Type of publication (PDB): Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Ramonienė, Edita;Krokaitė, Edvina;Jocienė, Lina;Shakeneva, Dinara;Kupčinskienė, Eugenija
Title: Testing validity of cpDNA, rnDNA and SSR markers for evaluation of Lythrum salicaria L. populations
Is part of: Smart Bio : ICSB 2nd international conference, 3-5 May 2018, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2018
Extent: p. 248-248
Date: 2018
Keywords: Lythrum salicaria L;SSR markers;DNA
ISBN: 9786098104486
Abstract: Higher soil concentrations of sodium chloride might be reflection of natural edaphic features and consequence of man-related activities. Near the rivers with elevated soil salinity, more intensive growth of Lythrum salicaria L. is well-known phenomenon. Anthropogenic activities, such as species introduction to the new areas, might contribute to the shifts of genetic diversity of L. salicaria. In this context, L. salicaria as halophyte and invasive species, is an important model for evaluation of anthropogenic effects. Till now data concerning genetic diversity of this species in Europe are very limited. Thereby our study aimed at molecular evaluation of populations of Lythrum salicaria growing in the riverbanks of Lithuania. Successes of comparison of populations firstly depends on the DNA marker selection. There are many cases when markers applied for one species might work for the other species of the same family. Among Lythraceae family representatives, the widest scope genetic evaluations were done for Punica granatum. Simple sequence repeat, SSR markers have been created for the accessions of horticultural importance of this species. SSR primers of Punica granatum failed to amplify DNA of Lythrum salicaria populations of Lithuania. It was true for the conditions described in Punica granatum protocol also for the set of buffer type, cycle number and temperature-related modifications of polymerase chain reactions (PCR). In addition to SSR markers, attempts were made to select primers for sequencing some nrDNA and cpDNA fragments. PCR reaction failed for primer combinations like rps16.5R+rps16.50F, trnK.UUU.3R+trnK.UUU.3F, 126614_F+127383_R (55 °C), although some primers created on the bases of chloroplast DNA, showed amplification of Lythrum salicaria DNA. Furthermore, several pairs of nrDNA primers, ITS4_R, ITS5_F and ITS7_F, demonstrated positive results of PCR. [...]
Internet: https://eltalpykla.vdu.lt/handle/1/36324
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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