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Type of publication: conference paper
Type of publication (PDB): Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Juškaitytė, Erika;Jocienė, Lina;Krokaitė, Edvina;Paulauskas, Algimantas;Kupčinskienė, Eugenija
Title: Selection of codominant markers for investigation of genetic diversity of Echinocystis lobata (Michx.) Torr. et A. Gray populations
Is part of: Smart Bio : ICSB 2nd international conference, 3-5 May 2018, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2018
Extent: p. 238-238
Date: 2018
Note: Study was funded by Lithuanian Research Council, Grant number No. SIT-02/2015
Keywords: Cucurbitaceae;Wild cucumber;Invasive species;SSR
ISBN: 9786098104486
Abstract: Wild cucumber (Echinocystis lobata) – climbing annual herbaceous plant, growing in coastal habitats, riverbanks, lakesides, wetlands and wastelands. It is native in North America, but in many European countries is recognized as invasive species. Within last two decades E. lobata is very intensively spreading in Lithuania and is considered to be one of the most aggressive invasive plants. Until now there is a lack of information about genetic diversity of Wild cucumber populations. Simple Sequence Repeats (SSR) markers appeared to be the most often used markers for understanding relations between invasive species and components of natural ecosystems. The objective of present study was to select codominant SSR markers for evaluation of genetic diversity of Echinocystis lobata populations. The DNA was extracted by DNA Purification Kit. Thirty SSR markers (N1, N6, N12, S9, S13, S15, S26, SSR10018, SSR05723, SSR16695, SSR16226, SSR23220, SSR22653, SSR23370, SSR01738, SSR16056, SSR05012, SSR05125, SSR07543, SSR19998, SSR16068, SSR02895, SSR20852, SSR31399, SSR20218, SSR29620, SSR14861, SSR11340, SSR11043) were tested for buffer type, temperature and other conditions of DNA amplification. DNA fragments were evaluated after electrophoresis on 1.5 % agarose gel. In our study seventeen (N6, N12, S9, S13, S15, S26, SSR05723, SSR23220, SSR23370, SSR05012, SSR07543, SSR19998, SSR16068, SSR31399, SSR20218, SSR29620, SSR11340) out of thirty markers showed positive results
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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