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Type of publication: conference paper
Type of publication (PDB): Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Šepetytė, Kamilė;Sakalauskaitė, Sandra;Daugelavičius, Rimantas
Title: Efflux pumps inhibition of Listeria monocytogenes cells
Is part of: Smart Bio : ICSB 2nd international conference, 3-5 May 2018, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2018
Extent: p. 161-161
Date: 2018
Note: This study was supported by Research Council of Lithuania, funding grant No MIP-40/2015
Keywords: Listeria monocytogenes;Efflux pumps inhibitors;L. monocytogenes cells
ISBN: 9786098104486
Abstract: Introduction: Listeria monocytogenes is an opportunistic foodborne Gram-positive pathogen causing serious human infections. These bacteria are widely distributed in the environment and are the third most common cause of death from food poisoning. Because of efflux pumps L. monocytogenes is a multidrug resistant pathogen, not sensitive to many antimicrobial compounds, including tetracycline, amoxicillin, benzalkonium chloride. Efflux pumps are membrane transporters which can extrude out of the cells various toxic compounds. Knowledge about the regulation of activity of these pumps could enhance the efficiency of treatment. One of ideas how to control the efflux is to use the pump inhibitors. Aim: To assessthe influence of potential inhibitors on the interaction of L. monocytogenes cells with efflux indicator Tetraphenylphosphonium (TPP+) ions and compare these results with the results of fluorimetric analysis. Materials and methods: TPP+ is universal efflux pump substrate in prokaryotic and eukaryotic cells. Electrochemical analysis was used to follow the distribution of TPP+ ions between the cells and the incubation medium. We used well-known inhibitors of Gram-positive cell transporters, such as Chlorpromazine, Reserpine and Verapamil, to analyze the efflux activity in L. monocytogenes. In addition, we explored effects of Phe-Arg-β-naphthylamide (PAβN), which is known as an inhibitor of RND family pumpsin Gram-negative bacteria. Fluorescence measurements were made with other efflux pumps substrate Ethidium to assay the intensity of their activity.[...]
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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