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Type of publication: conference paper
Type of publication (PDB): Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Degutytė-Fomins, Laima;Žūkienė, Rasa;Paužaitė, Giedrė;Pančenka, Olegas;Jarušaitis, Ainis;Naučienė, Zita;Mildažienė, Vida;Koga, Kazunori;Shiratani, Masaharu
Title: Cold plasma treatment as seed dormancy breaking agent
Is part of: Smart Bio : ICSB 2nd international conference, 3-5 May 2018, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2018
Extent: p. 128-128
Date: 2018
Keywords: Cold plasma;Seeds treatment;Raphanus sativus
ISBN: 9786098104486
Abstract: The aim of this study was to evaluate the dependence of atmospheric cold plasma (CP) irradiation effects on the duration of treatment and time interval passed between treatment and sowing. We estimated changes induced in radish (Raphanus sativus) germination and content of the main phytohormones controlling germination – abscisic acid (ABA) and gibberelins (GA7 and GA3) in dry seeds. Radish seeds were treated for different durations (1, 3, 5, 7 and 10 min) with a scalable dielectric barrier discharge (DBD) device. The discharge voltage, current and power density were 7.0 kV, 0.2 A and 3.1 W/cm2, respectively. Germination tests in vitro were performed 0, 1, 4, 8 days (CP 5 min) and 14 days (CP 1, 2, 3, 5, 7, 10 min) after treatment, and at the same time methanol extracts of dry seed homogenates were prepared for phytohormone HPLC analysis. Indices of germination dynamics – the final germination percentage Vi (%) and the median germination time Me (h), were determined using Richards plots. Morphometric seedling analysis was performed 4 days after sowing. CP treatment strongly stimulated germination: Vi increased from 18 to 30%; Me decreased from 15 to 29% in all CP treated seed groups in comparison to control. Increase of irradiation time above 5 min did not lead to stronger positive effects on germination as it was achieved by 5 min irradiation. Both effects on germination and morphometric parameters of seedlings were stronger when time interval between seed treatment and sowing was increased from 0 or 1 day to 4 days. In all groups of CP-treated seeds ABA content was strongly reduced while GA7 content was increased. Strong negative correlation (R2=0,934) was obtained between Vi and ABA content, and between Vi and ABA/(GA7+GA3) (R2=0,936). Positive polynomial correlation between Me and GA7+GA3 was characterized by R2=0,843. [...]
Affiliation(s): Biochemijos katedra
Botanikos sodas
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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