Antioxidants effect on MX-1 cells resistance

Abstract

Background. Multidrug resistance (MDR) is simultaneous resistance of cancer cells to many drugs used for chemotherapy. The main mechanism of MDR is multidrug resistance efflux pumps. It is a large superfamily of proteins called the ATP-binding cassette (ABC) transporters. The main efflux pumps responsible for MDR are P-glycoprotein (P-gp), multidrug-resistance protein (MRP) and breast cancer resistance protein (BCRP) (1). In this study we explored antioxidants inhibition activity to efflux pump. The aim: to investigate the effects of antioxidants on breast cancer cell line. Methods. The levels of MX-1 cell resistance to rutin (R), quercetin (Q) and epigallocatechin gallate (EGCG) were estimated using MTT viability test. The MDR pump functional activity was determined by fluorometric measurements. Results. In this study was derived a new MX-1 cells sublines resistance to rutin, quercetin and epigallocatechin gallate. These cells sublines showed different functional activity of the MDR pumps. Conclusion. All antioxidants on MX-1 cells affected differently. Quercetin increased the functional activity of the pumps, while epigallocatechin gallate suppressed.